Hemaloxyl'ni Staining Procedures 141 



Differentiator: 



70% ethyl alcohol 85.0 ml. 



formalin l.'5.0 ml. 



glacial acetic acid 15.0 drops. 



procedure: 



1. Deparaffinize and hydrate slides to water (remove HgCl2 if pres- 

 ent). 



2. Stain in scarba red: 5 minutes. 



3. Rinse in distilled water. 



4. Transfer to 70% alcohol: 2-3 minutes. 



5. Differentiate until nuclei are clear and sharp against colorless 

 backgroiuid. 



f). Dehydrate, 95% and absolute alcohol. 

 7. Clear in xylene and mount. 



results: 



chromatin and calcium — red 



