Mallory Staining 149 



[a) celestin blue: 2 minutes 



celestin blue B, C.l. 51050 0.5 gm. 



iron alum 5.0 gm. 



distilled water 50.0 ml. 



Dissolve alum in water, add celestin blue: boil 



3 minutes; 

 add glycerol 14.0 ml. 



{b) hematoxylin: 2 minutes 

 (r) rimning water: 5 minutes 

 (d) proceed to step 2 



Krichesky {1931) proposed the following for solution (Mallory) II. 



A. aniline blue, WS, C.l. 42780 2.0 gm. 



distilled water 100.0 ml. 



B. orange G, C.L 16230 1.0 gm. 



distilled water 100.0 ml. 



C. phosphomolybdic acid 1.0 gm. 



distilled water 100.0 ml. 



Keep solutions A, B and C in separate bottles because the mixture 

 deteriorates on standing. When ready to use, mix equal parts of each. 



Azan Stain, Mallory Heidenhain's (modified after koneff, 1938) 



fixation: Zenker-formol; other general fixatives fair, but following 

 them is improved by mordanting slides overnight in 3% potassium 

 dichromate. 



solutions: 



Azocarmine: 



azocarmine G, C.l. 50085 0.2-1.0 gm. 



distilled water 100.0 ml. 



glacial acetic acid 1 .0 ml. 



Boil azocarmine in water 5 minutes, cool, filter and add acetic acid. 



Aniline alcohol: 



aniline 1 .0 ml. 



85-90% ethyl alcohol 1000.0 ml. 



Acid alcohol: 



glacial acetic acid 1 .0 ml. 



90-95% ethyl alcohol 100.0 ml. 



