Mallory Staining 151 



acidophil cytoplasm — orange-red 

 chromophobes — colorless or light gray 



comments: 



The azan method is recommended particularly tor pituitary and pan- 

 creas but also is outstanding for connective tissue. If a tissue does not 

 "take" the azocarmine, it may be due to formalin content of the fixa- 

 tive. To correct this condition, mordant the sections overnight in 3% 

 aqueous potassium dichromate. 



Romeis {1948) suggests the substitution of acid alizarine blue for 

 azocarmine; this shortens the time and does not require heat. 



procedure: 



1. Stain in acid alizarine blue: 5 minutes. 



aluminum sulfate, AL,(S04);^ 10.0 gm. 



acid alizarine blue, C.I. 58610 0.5 gm. 



distilled water 100.0 ml. 



Brino; to boil: 5-10 min. Let cool. Fill to 100 ml. with distilled 

 water. Filter. Red violet color. 



2. Wash, riuining water. 



3. Treat with phosphotungstic or phosphomolybdic acid, 5% aque- 

 ous: 30 minutes. 



4. Wash in distilled water. 



5. Proceed to aniline blue stain. 



results: 



After phosphotungstic acid, the alizarine color turns red, stays in nu- 

 clei and muscles, but is removed from collagen. 



After phosphomolybdic acid, the nuclei and muscle will be blue. 



Mallory- Heidenhain Stain: Rapid One-Step Method (cason, 1950) 



fixation: any good general fixative. 



solution: 



Staining solution: 



distilled water 200.0 ml. 



Dissolve each of below before adding next stain: 



phosphotungstic acid 1.0 gm. 



orange G, C.I. 16230 2.0 gm. 



aniline blue, WS, C.I. 42780 1.0 gm. 



acid fuchsin, C.I. 42685 3.0 gm. 



Keeps for several months. 



