152 Conneclivc Tissue (chap. \?^) 



procedure: 



1. DeparafFinize and hydrate slides to water, remove HgCi2. 



2. Stain: 5 minutes. 



3. Wash in running water: 3-5 seconds. 



4. Dehydrate rapidly, clear, and mount. 



RESULTS : 



collaoen — blue 



sround substance of cartilage and bones — shades of blue 



mucus, amyloid, other hyaline substances — shades of blue 



nuclei, fibroglia, myoglia, neuroglia fibrils, axis cylinders, fibrin — red 



erythrocytes, myelin — yellow 



elastin — pale pink or yellow 



Tiichrome Staining 



Masson Trichrome Stain 



Masson (1928), when he developed this method, called it a trichrome 

 stain, although it included four dyes. Since then the trichrome name 

 has been applied to many modifications of Masson's method using many 

 combinations of different dyes. 



fixation: any general fixative. 



solutions: 

 Iron alum: 



ferric ammonium sulfate 4.0 gm. 



distilled water 100.0 ml. 



Masson A: 



acid fuchsin, C.I. 42685 \% aqueous 10.0 ml. 



ponceau de xylidine, C.I. 16150 1% aqueous . . 90.0 ml. 



glacial acetic acid 1.0 ml. 



Lillie (1940) suggests alternate stains for ponceau de xylidine: pon- 

 ceau 2R, nitazine yellow, Biebrich scarlet, azofuchsin 3B, G and -fG, 

 Bordeaux red, chromotrope 2R, chrysoidin, eosin Y, orange G and 

 crocein. 



Masson B: 



phosphomolybdic acid, 1% aqueous (0.5 gm./ 



50 ml. water) 50.0 ml. 



phosphotungstic acid, 1% aqueous (0.5 gm./ 



50 ml. water) 50.0 ml. 



