154 Connective Tissue (chap. 13) 



Masson Trichrome Stain, Modified (gurr, 1956) 

 fixation: any general fixative. 



solutions: 

 Iron alum: 



ferric ammonium sulfate 4.0 gm. 



distilled water 100.0 ml. 



Hematoxylins, see page 124. 

 Acid fuchsin: 



acid fuchsin, C.I. 42685 1.0 gm. 



distilled water 100.0 ml. 



glacial acetic acid 1.0 ml. 



Ponceau de xylidine: 



ponceau de xylidine, C.I. 16150 1.0 ml. 



distilled water 100.0 ml. 



glacial acetic acid 1.0 ml. 



Fast green: 



fast green FCF, C.I. 42053 2.0 gm. 



distilled water 100.0 ml. 



glacial acetic acid 2.0 ml. 



Phosphomolybdic acid: 



phosphomolybdic acid 1.0 gm. 



distilled water 100.0 ml. 



Acidic water: 



glacial acetic acid 1.0 ml. 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water, remove HgClo. Mor- 

 dant formalin-fixed tissue in saturated aqueous HgClo: 5 min- 

 utes. Wash in running water: 5 minutes. Treat with Lugol's and 

 sodium thiosulfate. 



2. Mordant in iron alum: | hour. 



3. Wash in running water: 5 minutes. 



4. Stain in hematoxylin (Delafield's or like): | hotir. 



5. Wash, running water: 5 minutes. 



6. Differentiate in saturated aqueous picric acid. 



7. Wash thoroughly in running water: 10 minutes or longer. 



8. Stain in acid fuchsin: 5 minutes. 



