TricJiromc Staining 155 



9. Rinse in distilled water until excess stain is removed. Check un- 

 der microscope. 



10. Stain in ponceau de xylidine: 1-5 minutes. 



11. Rinse in tap water, control under microscope for proper intensity 

 of acid fuchsin and ponceau de xylidine. 



12. Differentiate in phosphomolybdic acid: 5 minutes. 



1 3. No rinse, transfer directly into fast green: 2 miniues. 



14. Differentiate fast green in acidic water and dehydrating alcohols. 



15. Dehydrate in absolute alcohol, 2 changes: 3 minutes each. 



16. Clear and moinit. 



results: 



nuclei — deep mauve blue to black 



cytoplasmic elements — varying shades of red and mauve 



muscle — red 



collagen, mucus — green 



comments: 



Among the many modifications of Masson's stain, this is one of the 

 best, because it seems to offer good control of both red dyes by the 

 use of two separate solutions. In most cases, the full 5 minutes in the 

 acid fuchsin is advisable, but time in the ponceau de xylidine is not 

 quite so critical. 



Pollak's Rapid Method (1944) 



fixation: any general fixative. Formalin-fixed tissues are improved by 

 treating slides overnight with saturated aqueous HgCU. 



solutions: 



Hematoxylins, see page 124. 

 Trichrome stain: 



acid fuchsin, C.I. 42685 0.5 gm. 



ponceau 2R, C.I. 16150 1.0 gm. 



light green SF, yellowish C.I. 42095 0.45 gm. 



orange G, C.I. 16230 0.75 gm. 



phosphotungstic acid 1.5 gm. 



phosphomolybdic acid 1.5 gm. 



glacial acetic acid 3.0 ml. 



50% ethyl alcohol up to 300.0 ml. 



Add acetic acid to alcohol; split solution in 4 parts. Dissolve acid 

 fuchsin and ponceau in one part; light green in the second part; 

 orange G and phosphotungstic acid in the third part; and piiospho- 



