15G Collective Tissue (chap. 13) 



molybdic acid in the fourth part. Mix four sohuions and fiher. Keeps 

 well. 



Acidified water: 



glacial acetic acid 0.2 ml. 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgCU. 



2. Stain in Mayer's hematoxylin: 10 minutes. (Pollak uses Weigert's: 

 10-20 minutes.) 



3. Wash in running water: 10 minutes, 



4. Stain in trichrome stain: 7 minutes. 



5. Rinse briefly in distilled water. 



6. Differentiate in acidified water: only a few seconds; check under 

 microscope if necessary. 



7. Dip a few times in 70% alcohol. 



8. Dehydrate in 95% alcohol: several seconds in each of two changes. 



9. Dehydrate, absolute alcohol, clear and mount. 



results: 



nuclei — dark blue 

 muscle, elastin — red 

 fibrin, calcium — purple 

 hyalin — pale blue 

 collagen, mucus — blue green 



Gomori (1950B) Method 



fixation: any general fixative. Formalin-fixed tissues are improved by 

 treating slides overnight with saturated aqueous HgClo. 



solutions: 



Hematoxylins, see page 124. 

 Trichrome mixture: 



chromotrope 2R, C.I. 16570 0.6 gm. 



fast green FCF, C.I. 42053 or light green SF, C.I. 



42095 0.3 gm. 



phosphotungstic acid 0.6-0.7 gm. 



glacial acetic acid 1.0 ml. 



distilled water 100.0 ml. 



Keeps indefinitely. Aged solutions stain less red and more green than 

 fresh ones. 



