Trichrome Slainiyig 159 



muscle — red 



collagen, basement membrane— brilliant blue 



fibrin, serum protein — red 



red blood cells, keratin — bright red 



colloid, hyalins — blue to purple to red 



After HgCU: 



elastic fibers and mucus — purple 



Picro-Gomori Method (menzies, 1959) 



fixation: mercuric chloride type best. 



solutions: 



Hematoxylins, see page 124. 

 Orange G-picric acid: 



orange G, C.I. 16230 1.0 gm 



picric acid, saturated aqueous 100.0 ml. 



Light green-chromotrope: 



light green SF, yellowish, C.I. 42095 0.3 gm. 



phosphotungstic acid 0.6 gm. 



chromotrope 2R, C.I. 16570 0.6 gm. 



glacial acetic acid 1.0 ml. 



distilled water 99.0 ml. 



Acidic water: 



glacial acetic acid I.O ml. 



distilled water 100.0 ml. 



procedure: 



1 . Deparaffinize and hydrate slides down to water. Remove HgCU. 



2. Stain hematoxylin (Weigert's, Mayer's, etc.) for correct time de- 

 pending on hematoxylin used. 



3. Stain in orange G-picric acid: 1 minute. 



4. Wash in running water until erythrocytes are yellow: 5-10 min- 

 utes. 



5. Stain in light green-chromotrope: 2 minutes. 



6. Sharpen stain in acidic water, 3 changes: 1 minute each. 



7. Rinse in 70% alcohol. 



8. Hydrate, clear and mount. 

 results: 



erythrocytes — brilliant orange yellow 

 fibrin — orange red or red 



