162 Connective Tissue (chap. 13) 



results: 



elastin — red brown 

 nuclei, basophilic granules — blue 

 cytoplasm, muscle fibers — violet 

 collagen, reticulum, basement membrane — green 

 erythrocytes, myelin sheaths, acidophilic granules, plasmosomes — or- 

 ange 



Movat's Pentachrome Stain (1955) 



fixation: formol-sublimate-acetic is best: 12-18 hours. 



mercuric chloride 4.0 gm. 



formalin 20.0 ml. 



distilled water 80.0 ml. 



glacial acetic acid 5.0 ml. 



Do not use a dichromate fixative; it diffuses the alcian blue staining. 

 If 10% formalin is used, after the sections are mounted on slides and 

 hydrated, post-fix them in formol-sublimate-acetic for 2-3 hours, or 

 overnight. 



solutions: 

 Alcian blue: 



alcian blue 8GS, C.I. 74240 1.0 gm. 



distilled water 100.0 ml. 



Filter, and add: 



glacial acetic acid 1.0 ml. 



Prepare fresh each time. 



Weigert-Hart resorcin-fuchsin: 



Weigert's resorcin-fuchsin (stock solution): 



basic fuchsin, C.I. 42500 2.0 gm. 



resorcinol 4.0 gm. 



distilled water 200.0 ml. 



Mix in a porcelain dish and after it comes to a boil cook for 1 minute. 

 A scum should form. Add 25.0 ml. of 29% aqueous ferric chloride. 

 Cool, filter, and leave precipitate on filter paper until dry. Cover and 

 leave overnight. Place precipitate in porcelain dish and add 200.0 ml. 

 of 95% ethyl alcohol. Bring to a boil on an electric hot plate. Add 4.0 

 ml. of concentrated hydrochloric acid (see Comment 1). Remove from 



