Tric/iroine Staining 163 



hot plate and bring volume to 200.0 ml. by adding 95% alcohol. The 

 solution keeps well. 



Working solution: 



70% ethyl alcohol 94.0 ml. 



Weigert's resorcin-fuchsin 5.0 ml. 



hydrochloric acid (see Comment 1) 1.0 ml. 



Weigert's hematoxylin, page 000. 



Woodstain scarlet-acid fuchsin: 



Stock solution A: 



woodstain scarlet N.S.'^ 0.1 gm. 



distilled water 99.5 ml. 



glacial acetic acid 0.5 ml. 



Stock solution B: 



acid fuchsin, C.I. 42685 0.1 gm. 



distilled water 99.5 ml. 



glacial acetic acid 0.5 ml. 



Working solution: 



Solution A 40.0 ml. 



Solution B 10.0 ml. 



Phosphotungstic acid: 



phosphotungstic acid 5.0 gm. 



distilled water 100.0 ml. 



Alcoholic saffron: 



saffron- 6.0 gm. 



absolute ethyl alcohol 100.0 ml. 



Place in tightly corked bottle to prevent hydration and place in incu- 

 bator, 58 °C for 48 hours before use. Store in an airtioht bottle. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgCL. 



2. Rinse thoroughly in distilled water. 



3. Stain in alcian blue: 15 to 30 minutes. 



4. Wash in running water: 3 minutes. 



5. Place in alkaline alcohol: 2 hours: (Add a few drops of ammonia 

 to 95% alcohol until pH. is over 8. This solution converts the 

 alcian blue into an insoluble pigment.) 



^ Edward Gurr, London, England. 



^ Safran de Gatinais, Giiibler, may be oljtainetl from Roboz Surgical Co.. Washington, 

 B.C. 



