168 Couneclive Tissue (chap. 13) 



Aldehyde-Fuchsin (gomori, 1950C) (modified by cameron and Steele, 

 1959) 



fixation: any good fixative, preferably without dichromate. Bouin's 

 and formalin produce a colorless background. 



solutions: 



Potassium permanganate, 0.3%: 



potassium permanganate 0.3 gm. 



distilled water 100.0 ml. 



sidfuric acid, concentrated 0.3 ml. 



Sodium bistdfite, 2.5%: 



sodium bisulfite 2.5 gm. 



distilled water 100.0 ml. 



Aldehyde-f uchsin : 



Add 1 gm. basic fuchsin, C.I. 42500, to 200 ml. boiling water: boil 1 

 minute. Cool and filter. Add 2 ml. concentrated hydrochloric acid 

 and 2 ml. paraldehyde. Leave stoppered at room temperattire. When 

 mixture has lost reddish fuchsin color and is deep purple (3-4 days), 

 filter it and discard filtrate. Dry precipitate on filter paper in an oven. 

 Remove and store in bottle. Makes about 1.9 om. To make staining 

 solution, dissolve 0.25 gm. in 50 ml. of 70% alcohol. Keeps at least 

 six months. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgClo. 



2. Oxidize in potassium permanganate: 1 minute. 



3. Rinse in distilled water. 



4. Bleach in sodium bistilfite initil permanganate color is removed. 



5. Wash in running water: 5 mintites. 



6. Transfer to 70% alcohol: 2 minutes. 



7. Stain in aldehyde fuchsin: 2-10 minutes. 



8. Wipe off back of slide and rinse in 95% alcohol. 



9. Differentiate in 95% alcohol until no inore aldehyde-fuchsin 

 comes out of sections. 



10. Dehydrate, absoltite alcohol; clear and mount, 



results: 



elastin — deep purple 



mast cells, chief cells of gastric mticosa, beta cells of pancreas, baso- 

 phils of pittiitary and some kinds of mticin also stain pinple. 



