170 Connectix>e Tissue (chap. \?>) 



secting needles on a slide, allow to slightly dry and immerse in fixati\e. 

 The tissue, in most cases, will remain adhering to the slide through 

 staining solutions. 



If histologic rings are not available for spreading tissue, cut thick 

 filter paper into circles of a desired diameter and about 6-7 mm. in 

 width. Place the circle of filter paper under the omentum or other tis- 

 sue to be spread and press against the tissue. Cut out a ring of the tissue, 

 just beyond the outer edge of the paper circle and place in fixative. 

 Carry filter paper and tissue through staining procedures; connecti\e 

 tissue stains can be applied as usual. Finally remove the paper by peel- 

 ing it off when the tissue is being placed on a slide preparatory to mount- 

 ins: vvith cover s:lass. 



Bits of loose connective tissue which are difficult to handle may be 

 dehydrated and infiltrated with nitrocellulose. Carefully spread the tis- 

 sue in a film of nitrocellulose on a slide, allow to slightly dry, and 

 harden in 70% alcohol. It can be stained in place on the slide as this 

 is carried throuoh solutions like a nitrocellidose section. The latter 

 method usually gives more uniform staining. 



Bone Staining 



Decalcified (romeis, 1948) 



fixation: formalin, followed by decalcification (see pages 24-26). 



solutions: 

 Thionin: 



thionin, C.I. 52000, saturated in 50% ethyl 



alcohol 10.0 ml. 



distilled water 100.0 ml. 



If the color does not set correctly, add 1-2 drops of ammonia to stain- 

 ing solution. 



Carbol-xylol, see page 410. 



procedure: 



1. Deparaffinize and hydrate slides to water. If sections tend to loosen, 

 treat with 0.5-1% nitrocellulose (page 60). 



2. Stain in thionin solution: 10 minutes. 



3. Wash, distilled water: 20 minutes, change several times. 



4. Treat with picric acid, saturated aqueous: I minute. 



5. Rinse in distilled water. 



