Bo)ie Staining 171 



6. Differentiate in 70% alcohol: 5-10 minutes or more, until no 

 more color comes off. 



7. Dehydrate in 95% alcohol, 2 changes: 3 minutes each, 



8. Dehydrate and clear in carbol-xylol: 5 minutes. 



9. Clear in xylene, and moimt. 



results: 



lacunae and canaliculi — bordered with bluish black 

 background — yellow 



comments: 



Since occasionally paraffin sections loosen from the slides, some tech- 

 nicians prefer nitrocellulose embedding, but this takes time. 



Hand Ground, Undecalcified Sections (enlow, 1954) 



procedure: 



1. If necessary to remove organic materials and fat, treat in follow- 

 ing manner: 



(a) Boil in soap solution: 3-4 hours; and wash in running water: 

 3-4 hours. 



(b) Suspend over either or chloroform: 36-48 hours. 



(c) Allow to thoroughly dry. 



2. Cut slices as thin as possible without cracking them. A jeweler's 

 saw is recommended. 



3. Grind on sharpening hones with finely powdered carborundum 

 or household cleansing powder. Keep surface wet with water. 

 Optical or metallurgical grinding and polishing equipment, if 

 available, can be used. The grinding can be done between two 

 stones, or the section can be held on a wet rubber or cork stopper 

 and ground against the hone. If this becomes difficult to manage, 

 glue the section on a slide with Duco cement, and continue to 

 grind. When almost thin enough, loosen from slide with acetone, 

 turn section over, glue it down, and grind opposite side. 



4. Polish off both sides with fine leather strop. 



5. Place in 95% alcohol: 5 minutes. 



6. Air dry. 



7. Place in plastic solution, agitate to liberate air bubbles. 



parlbidin 28.0 gm. 



butyl (or amyl) acetate 250.0 ml. 



Let stand until parloidin is completely dissolved. Stir thoroughly. 



