ISO Silver Impregnation I (chap. 14) 



results: 



Reticulum — dark violet to black 



del Rio-Hortega Method (mallory, 1938) 

 fixation: any good general fixative. 



solutions: 



Ammoniacal silver carbonate: 



To 10 ml. of 10% silver nitrate add 10 ml. of saturated lithium 

 carbonate, aqueous. Shake, allow precipitate to settle. Decant and 

 wash precipitate Avith distilled water 5 times. Add 25 ml. of distilled 

 water. Almost dissolve precipitate with ammonia (28% reagent), 

 drop by drop, with shaking. Allo^v a few grains of precipitate to 

 remain. Add 95% alcohol up to 100 ml. Filter. Warm uncovered at 

 50°C for 20 minutes. Ready for use. Can be reused, but heat to 50°C 

 and filter before doing so. Keep in brown bottle. 



Formalin solution: 



formalin 10.0 ml. 



distilled water 50.0 ml. 



Gold chloride: 



gold chloride 1% stock solution (1 gm./lOO ml. 



water) 12.5 ml. 



distilled water 50.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgCl2. 



2. Treat with potassium permanganate, 0.2% (0.2 gm./lOO ml. 

 water): 3 miniues. 



3. Wash in distilled water: 2 minutes. 



4. Bleach in oxalic acid, 5% (5 gm./lOO ml. water): 3 minutes. 



5. Wash thoroughly in distilled water, several changes: 10 minutes. 



6. Impregnate with silver solution in 37 °C oven: 15-30 minutes. 

 Do not expose to bright light. Keep metal instruments out of 

 solution. 



7. Rinse quickly in distilled water. 



8. Reduce in formalin solution: 3 minutes. 



9. Wash in distilled water: 3 minutes. 



10. Gold tone until yellow color turns to purplish gTey. 



1 1 . Rinse briefly in distilled water. 



12. Fix in sodium thiosulfate, 5% (5 gm./lOO ml. water): 3 minutes. 



13. Wash in running water: 5 minutes. 



