196 Silver Impregnation II (chap. 15) 



solutions: 

 Cresyl violet: 



cresyl violet-acetate 0.5 gm. 



distilled water 100.0 ml, 



Resin-xylene: 



mounting resin 50.0 ml. 



xylene 50.0 ml. 



procedure: 



1. Deparaffinize and hydrate to water; remove HgCl2. 



2. Stain in cresyl violet: 3-5 minutes. 



3. Rinse in distilled water, 2 changes: few seconds each. 



4. Dehydrate in 95% alcohol: 30 seconds. 



5. Dehydrate in absolute alcohol: 30 seconds. 



6. Transfer to xylene: 1 minute. 



7. Treat with resin-xylene: 2 minutes. 



8. Differentiate in absolute alcohol: 10-30 seconds. Check under 

 microscope. 



9. Clear and mount. 



results: 



Nissl substance — purple 



comments: 



Steps 7, 8, and 9 may be repeated several times until desired differen- 

 tiation is reached. 



Banny and Clark [1950) recommend Coleman and Bell for the best 

 cresyl violet for Nissl staining. 



Thionin (clark and sperrv, 1945) S 



fixation: Bouin's recommended; other's are satisfactory; Zenker's not 

 recommended. 



sectioning: 10 microns; thinner sections of no advantage. 



solutions: 



Lithium carbonate, 0.55%: 



lithium carbonate 5.5 gm. 



distilled water 1000.0 ml. 



Thionin: 



thionin, C.I. 52000 0.25 gm. 



0.057o lithium carbonate 100.00 ml. 



