Neurofibrils 107 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgCU. 



2. Treat with lithium carbonate solution: 5 minutes. 



3. Overstain in thionin: 5-10 minutes. 



4. Rinse in distilled water. 



5. Dip in 70% alcohol: few seconds. 



6. Dehydrate in butyl alcohol, 2 changes: 2-3 minutes in each, 



7. Clear and moinit. 



results: 



Nissl substance — bright blue 



comments: 



If differentiation is necessary, briefly rinse slides in 95% ethyl alcohol 

 (following step 5) and place in aniline, then in lithitmi carbonate 

 saturated in 95% alcohol. Proceed to step 6. 



Gallocyanin (einarson, 1951) S 

 fixation: any general fixative. 



sectioning: 10 microns. 



solution: 



gallocyanin 0.15 gm. 



chrome alum (chromium potassium sulfate) . . 5.0 gm. 



distilled water 100.0 ml. 



Dissolve chrome alum in warm water, add gallocyanin and boil 

 gently for 10 minutes. Keeps well for about 1 week. 



procedure: 



1. Deparaffinize and hydrate slides to Avater; remove HgCl2. 



2. Stain in gallocyanin: overnight. 



3. Wash in rtmning water: 5 minutes. 



4. Dehydrate, clear, and mount. 



results: 



Nissl substance — blue 



Neurofibrils 



Ramon y Cajal's Method (modified from favorsky, 1930) B 



fixation: cut slices perpendictilar to organ surface, about 5 cm. thick. 

 Place in 70% alcohol, plus .5% glacial acetic acid: 6 hours. 



