Nerve Fibers at id Endings 203 



Nerve Fibers and Endings 



Bodian Method (1936, 1937) S 

 fixation: Bodian recommends: 



formalin 5.0 ml. 



glacial acetic acid 5.0 ml. 



80% ethyl alcohol 90.0 ml. 



Not suitable are chromates, chromic and osmic acid, or mercuric 

 chloride. 10% formalin causes excessive staining of connective tissue. 



sectioning: paraffin method. 



solutions: 



Protargol solution: 



Protargol i 1.0 gm. 



distilled water 100.0 ml. 



Sprinkle Protargol on surface of water in a wide dish or beaker. Do 

 not stir, this is critical. Keep on hot plate at 37°C until Protargol is 

 dissolved. 



Reducing solution: 



hydroquinone 1.0 gm. 



sodium sulfite 5.0 gm. 



distilled water 100.0 ml. 



Gold chloride: 



gold chloride 1.0 gm. 



distilled water 100.0 ml. 



Acidified water: 



glacial acetic acid 0.5 gm. 



distilled water 100.0 ml. 



procedure: 



1 . Deparaffinize and hydrate slides to water. 



2. Impregnate in Protargol solution plus 5 gm. clean copper (wire, 

 shot or sheet, cleaned with 1 part HNO3/3 parts HCl) to 100 ml. 

 of solution, 37 °C: 12-24 hours. 



3. Wash in distilled water, several changes. 



4. Reduce: 5-10 ininutes. 



^ Roboz Suigical Instruinent Co., \\ ashingloii, D.C. 



