214 Silver Impregnalioyi II (chap. 15) 



comments: 



MargoUs and Pickett include methods for following luxol fast blue 

 with phosphotungstic acid-hematoxylin to differentiate neuroglia 

 from myelin, and oil red O to distinguish degenerating myelin from 

 normal myelin. 



Dziabis (1958) describes a method for gross brain sections. 



Luxol Fast Blue- Holmes Silver Nitrate (margolis and pickett, 1956) S 

 fixation: 10% formalin preferred. 



embedding: paraffin method. 



solutions: 



Silver nitrate, 20%: 



silver nitrate . . 20.0 gm. 



distilled water 100.0 ml. 



Boric acid: 



boric acid 12.4 gm. 



distilled water 1000.0 ml. 



Borax: 



borax 19.0 gm. 



distilled water 1000.0 ml. 



Impregnating fluid: 



In 500 ml. cylinder mix 55 ml. boric acid soliuion and 45 ml. borax 

 solution. Dilute to 494 ml. with distilled water. With pipette, add 1 

 ml. of 1% aqueous silver nitrate (1 gm./lOO ml. water). With another 

 pipette add 5 ml. of 10% aqueous pyridine (10 ml./lOO ml. water). 

 Mix thoroughly. 



Reducer: 



hydroquinone 1.0 gm. 



sodium sulfite (crystals) 10.0 gm. 



distilled water 100.0 ml. 



Can be used repeatedly, but only for a few days. 



Luxol fast blue — see page 212. 



procedure: 



1. Deparaffinize and hydrate sections to water. 



2. Treat with 20% silver nitrate in dark, room temperature: I hour. 

 Prepare impregnating fluid. 



