Blood Smears 223 



solutions: Wilcox {1943) 

 W'right-Gicmsa: 



Giemsa powder 2.0 gm. 



glycerol 100.0 ml. 



Heat in water bath, 55-60°C: 2 hours, stirring at intervals. Avoid ab- 

 sorption of moisture by covering month of flask with double thickness 

 of paper secured with rubber band. 



Add: aged Wright's staining solution (2.0 gm. 



per 1000.0 ml. methyl alcohol) 100.0 ml. 



Let stand overnight: add additional \\>ight's 



staining solution 800.0 ml. 



Filter; ready for use. 



Working solution: 



Wright-Giemsa stock solution 1 part 



Neutral distilled water (buffer) 9 parts 



Buffer, pn 7.0: 



Soliuion A: 



dibasic sodium phosphate, anhydrous 9.5 gm. 



distilled water 1000.0 ml. 



Solution B: 



monobasic potassium phosphate 9.7 gm. 



distilled water 1000.0 ml. 



Working Solution: 



Solution A 61.1 ml. 



Solution B 38.9 ml. 



distilled water 900.0 ml. 



procedure: 

 Short Method: 



1. Stain film: 10 minutes. 



2. Flush scum from top of solution with natural water to avoid 

 picking tip precipitate on slides. 



3. Remove slides to neutral distilled water: 1 minute. 



4. Dry slides standing on end. Do not blot. Mount. 



Long Method (a more brilliant stain): 



1. Stain: 45 mintues in dilution of 1 to 50 of neutral water. 



