224 Hematologic Elements and Related Issues (chap. 16) 



2. Flush off scum and transfer slides to neutral distilled water: 3-5 

 minutes. 



3. Dry as above. 



Note: Thin smear and thick film can be stained on same slide. Fix thin 

 portion in methyl alcohol for 1-2 minutes, taking care that thick film 

 does not come in contact with alcohol. Stain as above (short method), 

 but shorten washing time to 2 or 3 dips so thin smear is not too light; 

 this shorter washing time will leave a deeper background in the thick 

 film. 



modifications: 



Fenton and Innes (1945). 



Manwell {1945). 



Modified Wright's stain, Steil {1936). 



STAINING OLD BLOOD SMEARS: 



Old smears will stain more brilliantly if treated 5-10 minutes in al- 

 cohol-acetic solution — 10 drops glacial acetic acid to 60 ml. absolute 

 alcohol. 



Blood Tissue Elements and Inclusion Bodies ^ 



Giemsa Stain, Wolbach's Modification (mallory, 1944) 

 fixation: any good general fixative. 



solutions: 

 Acid alcohol: 



70O7 p lyl alcohol 100.0 ml. 



gl acetic acid 0.5-1.0 ml. 



Giems ck solution: 



G >a powder I.O gm. 



mt yl alcohol, neutral, acetone-free 66.0 ml. 



gly \.rol 66.0 ml. 



Work Giemsa powder into glycerol and place in 60°C oven for 2 

 hours. Add methyl alcohol and stopper tightly. 



Working solution: 



Stock Giemsa solution 2.5 ml. 



methyl alcohol 100.0 ml. 



distilled water 3.0 ml. 



» See p. 306. 



