Blood Tissue Elcinenls and f)i(hi.si()n Bodies 225 



Rosin stO( k solution: (C'.olophonium) 



white wood rosin 10.0 gm. 



absolute alcohol 100.0 ml. 



Working solution: 



rosin stock solution 5.0 ml. 



95% ethyl alcohol 40.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water. Remove HgClo. 



2. Treat in acid alcohol: 5 minutes (not necessary if tissue has been 

 subjected to acid decalcification). 



3. Wash, running water: 5 minutes. 



4. Rinse in distilled water. 



5. Place in freshly mixed Giemsa working solution: 1 hour. 



6. Transfer to second iar of Giemsa w'orkino solution: overnioht. 



7. Rinse in distilled w-ater. 



<S. Differentiate in rosin alcohol. 

 9. Rinse in 95% alcohol. 

 10. Dehydrate in absolute alcohol, 2 changes; clear and mount. 



results: 



nuclei — reddish purple 



other tissue elements — similar to Wrig^ht's stain 



inclusion bodies — blue to purplish blue 



comments: 



For other stains for inckision bodies, see pages 326-328. 



Staining for Malaria 



Jenner-Giemsa (mcclung, 1939, modified may-grunwaI ^ 



fixation: Smears may be fixed during staining process. T -• sections 

 in any good general fixative, preferably containing mercLusj-'chloride 

 and alcohol. rijf 



solutions: "^"^ 



Jenner's solution: 



Jenner's stain 0.2 gm. 



methyl alcohol, neutral, acetone free 100.0 ml. 



Giemsa stock solution: 



Giemsa powder 3.8 gm. 



methyl alcohol, neutral, acetone free 75.0 ml. 



glycerol 25.0 ml. 



