226 Hematologic Elements and Related Issues (chap. 16) 



"W^ork stain into glycerol, warm for 2 hours, 60°C oven. Add methyl 

 alcohol. 



Working solution: 



Giemsa stock solution 10.0 ml. 



distilled water 100.0 ml. 



or 1.0 ml. to 10.0 ml. water for flooding a slide. 



procedure: 



1. Deparaffinize sections and run down to 50% alcohol. (Remove 

 HgClo, if present.) 



2. (rt) Sections — flood with ample amoimt of Jenner's sohuion and 

 add an equal amount of distilled water, or mix equal amoiuits of 

 Jenner's solution and water and flood slide or stain slide in coplin 

 jar: 4 minutes. 



{b) Smears — flood with Jenner's solution: 3 minutes. Add equal 

 amount of distilled water: 1 minute. 



3. Potir off Jenner's; no rinse. 



4. Flood with diliued Giemsa solution or place in coplin jar of 

 solution: 15-20 minutes. 



5. Rinse off stain with distilled water and continue to differentiate 

 with distilled water. If too blue, eosin color can be brought out by 

 rinsing in 1% acetic acid in water. 



6. Dehydrate in following sequence of acetone and xylene. Do not 

 use alcohol; it extracts the stain. 



acetone 95 parts: xylene 5 parts 

 acetone 70 parts: xylene 30 parts 

 acetone 50 parts: xylene 50 parts 

 acetone 30 parts: xylene 70 parts 

 acetone 5 parts: xylene 95 parts 



7. Clear in xylene and moimt. 



results: 

 malaria: 



chromatin — red or ptirplish red 



cytoplasm — blue (other elements' color similar to Wright's stain) 

 pigment — yellow brown to black 

 Schiiffner's stippling — -red 



comments: 



A beautifid clear stain for any blood picture, particularly blood 

 parasites. 



