Bone Mnrroiu Staining 229 



tissue receptacle and carry through fixation, washing, dehydraticjn 

 and infiltration without removing. No marrow will be lost. 



Minute amoimts of marrow may prove difficult to recover from 

 the filter paper without loss of material. The author has avoided 

 loss by allowing the paraffin around the marrow (on the filter pa- 

 per) to congeal slightly. Then carefully scrape paraffin and marrow 

 together and remove to melted paraffin in mold. The congealed 

 paraffin containing the marrow ^vill sink to the bottom of the mold 

 or can be pressed down into desired position. This method keeps 

 the marrow concentrated reasonably well. 



4. Glide and Odell (195'>) recommend for dilution vinisil (Abbott 

 Laboratories), a 3.5% sokition of polyvinylpyrrolidone (PVP) in 

 normal saline. 



Endicott (1945) used plasma seriun as a diluting fluid to thin 

 smears. The serum can be kept on hand for several months if 

 stored in refrigerator. 



5. For a fluorescent method, see Werth (1953). 



Phloxine-Methylene Blue (thomas, 1953) 

 fixation: any general fixative. 



solutions: 



Phloxine solution: 



phloxine B, C.I. 45410 0.5 gm. 



distilled water 100.0 ml. 



glacial acetic acid 0.2 ml. 



A slight precipitate will form. Filter before use. (The author has 

 foiuid this of no importance; the precipitate settles at bottom of 

 bottle and does not collect on the tissue.) 



Methylene blue-azure solution: 



methylene blue, C.I. 52015 0.25 gm. 



azure B, C.I. 52010 0.25 gm. 



borax 0.25 gm. 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgClo. 



2. Stain in phloxine: 1-2 minutes. 



3. Rinse well in distilled water. 



