230 Hematologic Elements and Related Issues (chap. 16) 



4. Stain methylene blue-azure: 0.5-1 minute. 

 .5. Partially destain in 0.2% aqueous acetic acid. 



6. Complete differentiation in 95% alcohol, 3 changes. 



7. Dehydrate, absolute alcohol; clear and mount. 



results: 



nuclei — blue 



plasma cell cytoplasm — blue 



other tissue elements — shades of rose and red 



comments: 



Thomas substitutes azure B for azure II of other methods because of 

 the uncertain composition of the latter. It is unnecessary to use 

 colophonium alcohol as in other methods. The author recommends 

 Thomas' method over all others she has tried. It is practically fool- 

 proof. 



Delez and Davis (1930) make up their phloxine with oxalic acid; 

 1% phloxine in 0.05% aqueous oxalic acid. 



This staining method frequently is used for general staining in 

 place of a hematoxylin-eosin method. 



Staining for Fibrin 



Ledrum's Acid Picro-Mallory Method (culling, 1957) 

 fixation: any good general fixative. 



solutions: 



Celestin blue: 



celestin blue B, C.I. 51050 0.25 gm. 



ferric alum, 5% (5 gm./lOO ml. water) 50.0 ml. 



Boil 3 minutes. Cool and filter. Add: 



glycerol 7.0 ml. 



Keeps for several months. 



Mayer's hematoxylin, see page 125. 



Picro-orange: 



picric acid saturated in 80% alcohol 100.0 ml. 



orange G, C.I. 16230 0.2 gm. 



