Staini?ig for Fibrin 231 



Acid fuchsin: 



acid fuchsin, C.I. 42685 1.0 gm. 



distilled water 100.0 ml. 



trichloracetic acid 3.0 gm. 



Phosphotiingstic acid: 



phosphotungstic acid 1.0 gm. 



distilled water 100.0 ml. 



Aniline blue: 



aniline blue, WS, C.I. 42780 2.0 gm. 



distilled water 100.0 ml. 



glacial acetic acid 2.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgClo. 



2. Stain in celestin blue: 3-5 minutes. 



3. Rinse in tap water. 



4. Stain in Mayer's hematoxylin: 5 minutes. 



5. Wash in running water: 5 minutes. 



6. Rinse in 95% alcohol. 



7. Stain in picro-orange: 2 minutes. 



8. Stain in acid fuchsin: 5 minutes. 



9. Rinse in distilled water. 



10. Dip in equal parts of picro-orange and 80% alcohol: few seconds. 



11. Differentiate in phosphotungstic acid: 5-10 minutes, until colors 

 are clear. 



12. Rinse in distilled Avater. 



13. Stain in aniline blue: 2-10 minutes. 



14. Rinse in distilled water: 2-3 minutes. 



15. Dehydrate; clear and mount. 



results: 



fibrin — clear red 

 erythrocytes — orange 

 collagen — blue 

 nuclei — blue-black 



comments: 



This method is specific for fibrin, setting it off sharply from other 

 tissue elements. See also Phosphotungstic acid-Hematoxylin (page 

 136), and Gram-Weigert (page 307). 



