240 Pignioits cuid Minerals (chap. 17) 



Bile Pigment Staining 



Click (1949) 



fixation: formalin or alcohol: approximately 6 hours. 



solutions: 



Tincture of iodine: 



iodine 10.0 gm. 



potassium iodide 6.0 gm. 



distilled water 5.0 ml. 



95% ethyl alcohol 95.0 ml. 



Lugol's soliuion: 



iodine 1.0 gm. 



potassium iodide 2.0 gm. 



distilled water 100.0 ml. 



Dissolve potassium iodide in water first, then add iodine. 



Stein's working solution: 



tincture of iodine 10.0 ml. 



Lugol's solution 30.0 ml. 



Sodium thiosulfate, 5%: 



sodium thiosulfate 5.0 gm. 



distilled water 100.0 ml. 



Safranin, see page 233. 



procedure: 



1. Deparaffinize and hydrate slides to water. 



2. Treat with Stein's iodine solution: 6-12 hours. 



3. Wash in distilled water: 5-10 mintUes. 



4. Bleach in sodium thiosulfate: 5-10 minutes. 



5. Wash in distilled water: 2-3 mintUes. 



6. Counterstain in safranin (or other red nuclear stain): 2-3 minutes. 



7. Rinse in 70^^ alcohol. 



8. Dehydrate, clear, and mount. 



results: 



bile pigment — emerald green 

 nticlei — red 



