Chapter J_§ 



Cytoplasmic 

 Elements 



Protein Staining 



Protein demonstration is of minor importance, but sometimes it 

 becomes necessary to determine the protein or nonprotein nature of 

 Granulation in cells. The Millon reaction is one of the oldest and most 

 reliable methods but actually is specific for phenols and for the one 

 amino acid, tyrosine. 



Millon Reaction (gomori, 1952) 

 fixation: any good general fixative. 



solutions: 



A. mercuric acetate 5.0 gm. 



distilled water 100.0 ml. 



trichloracetic acid 15.0 gm. 



B. sodium nitrite, 1% aqueous 10.0 ml. 



procedure: 



1. Run sections down to water. (Cut sections at least 10 microns 

 thick.) 



2. Incubate sections in solution A, 30-37 °C; 5-10 minutes. 

 .'». Add solution B: incubate another 25 minutes. 



4. Rinse in 70% alcohol. 



5. Dehydrate, clear and moiuit. 



24G 



