Golgi Apparatus and Mitochondria 259 



Ethylene glycol solution (or propylene glycol), 85%: 



ethylene glycol 85.0 ml, 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and transfer slides into absolute alcohol: 2 minutes. 



2. Transfer to absoltuc ethylene glycol. 



3. Stain in Stidan black solution: 30 minutes. 



4. Differentiate in 85% ethylene glycol: 2-3 minutes. 



5. Wash in distilled water. 



6. Counterstain, if desired. 



7. Wash well, mount in Apathy, or in glycerol jelly. 



results: 



phospholipids — black 



comment: 



Phospholipids are finding increasing popularity in the study of mito- 

 chondria and Golgi apparatus, and are an important constituent of 

 myelin. 



Golgi Apparatus and Mitochondria 



The Golgi apparatus (Golgi bodies, Golgi substance, Golgi complex) 

 usually is lost in routine fixation and requires special treatment. Since 

 the methods are not always predictable under all conditions, it may be 

 necessary to modify the fixing and/or staining time before attaining 

 precise results. In osmium and silver methods the Golgi appears either 

 as a dark net, a granular mass, a cord, or even a more difftise condition. 

 These methods seem to indicate it is fatty in nature. 



Mitochondria are tiny bodies, granular, rod-like or filamentous in 

 shape and scattered through the cytoplasm. They are lipoprotein in 

 composition and are easily lost in routine preparation. It is therefore 

 imperative that small pieces of tissue be fixed rapidly in order to pre- 

 serve any mitochondria. 



Golgi Apparatus Staining 

 Osmium Tetroxide: Ludford's Method (lillie, 1954B; cowdrv, 1952) 



