Golgi Apparatus and Mitochondria 263 



4. Treat with sattirated picric acid, aqtieoiis: 10 minutes. 



5. Rinse in distilled water. 



6. Treat with phosphomolybdic acid: 1 minute. 



7. Rinse in distilled water: 1 minute. 



8. Stain in safranin: 3-6 minutes. 



9. Dehydrate, clear and mount. 



results: 



mitochondria — dark bltiish green 



erythrocytes, nucleoli, plasmosomes — grass green (only other elements 



staining green) 



Altmann's Method: (modified by cowdry, mcclung, 1939). 



fixation: Regaud solution (page 20): change every day for 4 days, in 

 refrigerator. 



Mordant in 3% potassium dichromate: 8 days, change every second 

 day. 



Wash in rtnining water: overnight; dehydrate and embed. 



solutions: 



Altmann's aniline fuchsin: 



Make a saturated solution of aniline in distilled water by shaking the 

 two together. Filter. Add 10 gm. acid fuchsin, C.I. 42685, to 100 ml. 

 of filtrate. Let stand for 24 hours. Good for only 1 month. 



Methyl green: 



methyl green, C.I. 42590 1.0 gm. 



distilled water 100.0 ml. 



procedure: 



1. Deparafhnize and hydrate slides to water. 



2. Treat with potassium permanganate, 1% (I gm./lOO ml. water): 

 30 seconds (see comments beloAv). 



3. Rinse briefly in distilled water and bleach in oxalic acid, 5% (5 

 gm./lOO ml. water): 30 seconds. 



4. Rinse in several changes distilled water: 1-2 minutes. 



5. Dry slide around sections with filter paper, cover with Altmann's 

 fuchsin. Heat gently until ftmies smelling of aniline come off. 

 Cool. Allow stain to remain 6 mintites. 



0. Dry off most of stain. Rinse in distilled water until sections only 

 hold stain. If a large amount is left in sections it will form a trou- 



