270 Cytoplasmic Elements (chap. 18) 



{n) 0.5% cetylpyridiiim chloride (CPC) in 4% aqueous formalin. 

 {b) 0.4% 5-aminoacridine hypo(hloride in 50% ethyl alcohol. 



4. Spicer and Meyer {I960) precede staining with alcian blue by 5 

 minutes in aldehyde-fuchsin (page 287); to 70% alcohol, running 

 water, and then into alcian blue. 



5. Mowry {I960) reports that lots of alcian blue have differed in 

 staining efficiency and fastness. Current stocks of alcian blue 8GX 

 are more soluble, less fast and more stal^le. He has changed his stain- 

 ing method: 0.5-2.0% acetic acid or in 0.1 M, pW 2.2.-2. A, hydro- 

 chloric acid-sodium citrate buffer: 1-2 hours. 



Colloidal Iron Method (ritter and oleson, 1950) 



fixation: 10% formalin in 90% alcohol; do not use dichromate — it 

 oxidizes polysaccharides. 



solutions: 



Colloidal iron solution: 



dialyzed iron, Merck's 50.0 ml. 



2M acetic acid (11.5 ml./88.5 ml. water) 50.0 ml. 



Potassium ferrocyanide in HCl: 



potassium ferrocyanide, 1% aqueous 86.0 ml. 



N HCl (page 408) 14.0 ml. 



Periodic acid: 



periodic acid 0.4 gm. 



distilled water 100.0 ml. 



M/5 sodium acetate (135 mg. hydrated salt/35 



ml. ethyl alcohol) 5.0 ml. 



Reducing rinse: 



potassium iodide 1.0 gm. 



sodium thiosulfate 1.0 gm. 



distilled water 20.0 ml. 



Add with stirring: 



absolute ethyl alcohol 30.0 ml. 



2N HCl (page 408) 0.5 ml. 



Sulfite rinse: 



water 50.0 ml. 



concentrated HCl 0.5 ml. 



potassium metabisulfite 0.2 gm. 



Schiff reagent (page 294) 



