274 Cytoplasmic Elements (chap. 18) 



solutions: 

 Iron alum: 



ferric ammonium sulfate 4.0 gm. 



distilled water 100.0 ml. 



Acridine orange: 



acridine orange, C.I. 46005 0.1 gm. 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and hydrate paraffin sections to water, or cut frozen 

 sections and place in distilled water. 



2. Treat with iron alum solution, 5-10 miniues. 



3. Wash briefly in distilled water. 



4. Stain in acridine orange, 1| miniues. 



5. Rinse briefly in distilled water, blot and mount in Harleco Fluo- 

 rescence Oil AlountantJ 



results: 



mucin — brilliant reddish orange fluorescence 



comments: 



This is not a permanent slide. The iron inhibits the production of 

 fluorescence with acridine orange in nearly all tissue components 

 except mucin. Hicks and Matthaei also stiggest that the mucins of acid 

 polysaccharides rather than mucoproteins fluoresce. 



For details concerning fluorescent microscope and eqtiipment, see 

 page 102. 



Amyloid Staining 



Higman's Congo Red: (modified by lillie, 1954) 

 fixation: 10% formalin or an alcoholic fixative. 



solutions: 

 Congo red: 



Congo red, C.I. 22120 0.5 gm. 



50% ethyl alcohol 100.0 ml. 



Potassium hydroxide: 



potassium hydroxide 0.2 gm. 



80% ethyl alcohol 100.0 ml. 



Hematoxylin, page 125. 



' Hartman Leddon Co., Philadelphia. 



