Amyloid Staiyiijig 275 



procedure: 



1. Deparaflinize slides, transfer through absolute, 95%, and into 50% 

 alcohol. 



2. Stain with Congo red: 1-5 minutes. 



3. Differentiate in potassium hydroxide: 1--5 minutes. 



4. Rinse in tap ^vater. 



5. Stain in Mayer's hematoxylin (or the like): 2-3 minutes. 



6. Wash in tap water: 5 minutes. 



7. Dehydrate, clear, and mount. 



results: 



amyloid — red 

 nuclei — blue 



comments: 



Amyloid is a hyaline material that appears in connective tissue as a 

 result of disease and methods should be on hand for pathological 

 study. 



See also Metachromatic Method, page 278. 



Fluorescent Method (vassar and culling, 1959) 



fixation: 10% formalin or alcohol. The latter gives a crisper differ- 

 ential picture. 



solutions: 

 Thioflavine: 



thioflavine T, C.I. 49005 1.0 gm. 



distilled water 100.0 ml. 



Acetic acid, 1%: 



glacial acetic acid 1.0 ml. 



distilled water 99.0 ml. 



procedure: 



1. Place frozen sections in distilled water. 



2. Stain sections in thioflavine: 3 minutes. 



3. Differentiate in 1% acetic acid: 10 minutes. 



4. Wash briefly, blot, and mount in Harleco Fluorescence Oil 

 Mountant,^ or Apathy Mountant (page 119). 



results: 



amyloid — yello^v fluorescence 



' Hartman Leddon Co., Pliiladelphia, 



