Mast Cell Staining Zll 



Bismarck Brown (spatz, 1960) 

 fixation: 10% formalin. 



solution: 



Bismarck brown: 



Bismarck brown, C.I. 21000 0.5 gm. 



absolute ethyl alcohol 80.0 ml. 



1% aqueous hydrochloric acid 20.0 ml. 



procedure: 



1. Deparaffinize and transfer sections to absolute alcohol: 3 minutes. 



2. Dip in 957o. then 70% alcohol. 



3. Stain in Bismarck brown: 30 to 90 minutes. 



4. Differentiate in 3 changes 70% alcohol: 2 seconds each. 



5. Transfer to 95% alcohol: 2 seconds. 



6. Dehydrate in absolute alcohol: 30 seconds. 



7. Clear in xylene: 1 second; moiuit. 



results: 



mast cell granules — yellow brown 



comments: 



If counterstaining is desirable, follow the 70% differentiation by 3 

 miniues in hematoxylin (Harris, Mayer's, etc.); dip in 70% alcohol: 

 2 seconds, and continue into step 5 above. 



ChrysoidLn (harada, 1957) 

 fixation: any general fixative. 



solution: 



chrysoidin Y, C.I. 1 1270 0.5 gm. 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and run slides down to 80% alcohol; remove HgCU 

 if present. 



2. Stain in chrysoidin: 5-10 minutes. 



3. Rinse in distilled water. 



4. Dehydrate, clear, and moimt. 



results: 



mast cell gTanules — deep brown to black 

 other elements — yellowish 



