Mrtnrhronmsw 279 



procedure: 



1. Run frozen sections or deparaffinized paraffin sections down to 

 water. 



2. Stain in w^orking solution: 5 minutes to 24 hours. 



3. Rinse in water. 



4. Moiuit in Abopo)}, page 122 



results: 



amyloid — purple 



other tissue elements including hyalin — blue 



comments: 



Acid in the staining solution makes it self-differentiating and staining 

 time is flexible. Lieb suggests that if there is only a small amoiuit of 

 amyloid in the tissue, thicker sections should be cut to make the color 

 reaction more clearly visible. Slides moimted in Abopon have re- 

 tained their color for 2 years. Abopon mounts mtist be sealed with 

 ringing cement for permanency. 



Gomori {1933) otitlines a simple method for permanent motints: 



1. Float paraffin sections on dye solution: 15-20 miniues. 



2. Float sections on distilled water to remove excess dye. 



3. Float on 1-2% acetic acid, aqueous, to differentiate. 



4. Moinit on slides in usual fashion and dry. 



5. Remove paraffin with xylene and mount. 



This preser\es the metachromasia of the crystal \'iolet. 



Mucin^^ 



Toluidine Blue (lillie, 1929) 



fixation: any general fixative, but alcohol preferred. 



solution: 



toluidine O, C.I. 52040 2.0 gm. 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgCU. 



2. Stain in toluidine blue: 1 minute. 



3. Wash in water: 2-3 minutes. 



4. Dehydrate in acetone, 2 changes: 3-5 minutes each. 



5. Clear in xylene and mount. 



^° See Mucin Staining, page 273. 



