280 Cytoplasmic Elements (chap. 18) 



results: 



mucin — reddish violet 



nuclei and bacteria — deep blue 



cytoplasm, fibrous tissue — bluish green 



bone — bluish green 



cartilage matrix — bluish violet 



muscle — light blue 



cell granules — blue violet 



hyalin and amyloid — bluish gTeen 



Thionin (mallory, 1938) 



fixation: any general fixative, but alcoholic preferred. 



SOLUTION : 



thionin, C.I. 52000 1.0 gm. 



25% ethyl alcohol 100.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgClo. 



2. Stain in thionin: 15 minutes to 1 hour. 



3. Differentiate in 95% alcohol. 



4. Dehydrate in absolute alcohol, clear, and moimt. 



results: 



mucin — light to dark red or purple 



Acid Mucopolysaccharides^^ 



Thionin (gurr, 1958) 



fixation: 10% formalin or other general fixative. 



solutions: 

 Thionin: 



thionin, C.I. 52000, saturated aqueous 0.5 ml. 



distilled water 100.0 ml. 



Molybdate-ferricyanide solution : 



ammonium molybdate, 5% aqueous 50.0 ml. 



potassium ferricyanide, 1% aqueous 50.0 ml. 



Make up solutions fresh each time. 



procedure: 



1. Deparaffinize and hydrate slides to water. 



See Acid Mucopolysaccharides under Saccharides, page 2G9. 



11 



