284 Cytoplasmic Elements (chap. 18) 



Five methods have been included here. For additional information 

 some of the following authors may be of assistance: Eljtman [1957 A; 

 1959 A and B; 1960); Gomori {1931); Kerenyi {1959); Landing {1954); 

 Landing and Hall {1955); Lazarus {1958); Paget and Eccleston {1959, 

 1960);Pearse {1949, 1950); Shanklin, Nassar, and Issidorides {1959). 



Characteristic staining of cells types is also used to differentiate the 

 various cells of the islets of Langerhans of the pancreas. Gomori's 

 {1941 B), method is recommended, but the Heidenhain Azan method 

 (page 149) also is excellent and brilliant. 



Trichrome PAS ^ (pearse, 1950) 

 fixation: Helly's is best. 



solutions: 



Periodic acid: 



periodic acid 0.4 gm. 



M/5 sodium acetate 5.0 ml. 



95% ethyl alcohol 35.0 ml. 



distilled water 10.0 ml. 



Reducing solution: 



potassium iodide 1.0 gm. 



sodium thiosulfate 1.0 gm. 



95% ethyl alcohol 30.0 ml. 



distilled water 20.0 ml. 



2N HCl (page 408) 0.5 ml. 



A precipitate of sulfur forms; do not filter out. Make fresh just before 

 use. 



Schiff's reagent, see page 294. 



Sulfite baths: 



sodium metabisulfite, Na2S205 0.5 gm. 



distilled water 100.0 ml. 



Celestin blue solution: 



celestin blue B, C.I. 51050 0.5 gm. 



ferric ammonium sulfate (iron alum) 5.0 gm. 



distilled water 100.0 ml. 



Dissolve alum in water, add celestin blue; boil 3 minutes. Cool and 

 filter. Add: 



glycerine 14.0 ml. 



^ Concerning PAS technics, see page 298. 



