Piluitary and Pancreas 287 



20. Dehydrate in absolute alcohol, 2 changes: 3-5 minutes each. 



21. Clear and mount. 



results: 



beta cell granules — pale yellow orange 



beta cell cytoplasm — pale yellowish green 



alpha cells — deep orange 



delta cells — translucent green 



glycogen, mucus, glycoproteins — dark red to purplish 



nuclei — black 



collagen — green 



PAS-positive elements — magenta 



erythrocytes — yellow 



Aldehyde fuchsin-PAS Method (elftman, 1959 A and B) 

 fixation: overnight or week end in: 



mercuric chloride, 5% aqueous 100.0 ml. 



potassium chromium sulfate 5.0 gm. 



formalin 5.0 ml. 



Prepare fresh. Wash in 70% alcohol. 



solutions: 



Aldehyde fuchsin: 



basic fuchsin, C.I. 42500 0.5 gm. 



707o ethyl alcohol 100.0 ml. 



paraldehyde 0.75 ml. 



h)clrochloric acid, concentrated 1.25 ml. 



Put in 37 °C oven early in morning, and will be ready by noon of 

 succeeding day. 



Schiff's reagent, see page 294. 



Orange G: 



orange G, C.I. 16230 3.0 gm. 



distilled water brought to pH 2.0 with few drops 



of glacial acetic acid or hydrochloric acid , 100.0 ml. 



procedure: 



1. Deparafhnize and run slides down to 70% alcohol: remove HgClo 

 in iodine-70% alcohol. 



2. Stain in aldehyde fuchsin: 30 minutes. 



3. Rinse in 95% alcohol. 



4. Oxidize in periodic acid, 1% (1 gm./lOO ml. water): 15 minutes. 



