Piliiilary and Pancreas 289 



chromophobe granules — slate grey to pale pink 

 colloid — red to bliie-v iolet 

 red cells — orange 

 collagen — blue 



Cameron and Steele (1959) Method 

 fixation: any good fixative. 



solutions: 



Potassium permanganate, 0.'5%: 



potassium permanganate 0.3 gm. 



distilled water lOO.O ml. 



sulfuric acid, concentrated 0.3 ml. 



Soditim bisulfite, 2.5%: 



sodium bisulfite 2.5 gm. 



distilled water 100.0 ml. 



Aldehyde-fuchsin, page 16(S and 287. 



Halini's mixture (1952): 



distilled water 100.0 ml. 



lightgreen SF, yellowish, C.I. 42095 0.2 gm. 



orange G, C.I. 16230 1.0 gm. 



chromotrope 2R, C.I. 16570 0.5 gm. 



phosphotungstic acid 0.5 gm. 



glacial acetic acid 1-0 ml. 



Keeps indefinitely. 



procedure: 



1. Deparalfinize and hydrate slides to water; remove HgCU. 



2. Oxidize in potassium permanganate: 1 mintite. 



3. Rinse in distilled water. 



4. Bleach in sodium bisulfite until permanganate color is removed. 



5. Wash in running water: 5 minutes. 



6. Transfer to 70% alcohol: 2 minutes. 



7. Stain in aldehyde-fuchsin: 2-10 minutes. 



8. Wipe off back of slide and rinse in 95% alcohol. 



9. Differentiate in 95% alcohol tuitil no more aldehyde-fuchsin 

 comes out of sections. 



10. Transfer to 70% alcohol: 2 minutes. 



11. Rinse in distilled w^ater: 1 minute. 



12. Stain in Halmi's mixture: 45 seconds. 



