200 Cytoplasmic Elements (chap. LS) 



13. Wipe off back of slide, differentiate in 95% alcohol plus 0.2% of 

 acetic acid: 2-3 minutes. 



14. Rinse in fresh 95% alcohol. 



15. Dehydrate in absolute alcohol, clear, and mount. 



results: 



granulation of beta cells — dark purple 



delta cells — green 



acidophilic granules — orange 



nucleoli — bright red 



coagulated contents of cytoplasmic granules — orange 



Pancreas 



Chromium- Hematoxylin-Phloxine (gomori, 1941 B) 



fixation: Bouin's preferred. Stieve's satisfactory. Zenker's, Carnoy, and 

 formalin unsatisfactory. 



solutions: 



Bouin's solution, see page 13. 

 Potassium dichromate-sulfuric acid: 



Can be made up as separate 0.3% solutions and mixed or: 



potassium dichromate 0.15 gm. 



distilled water 100.0 ml. 



sulfuric acid, concentrated 0.15 ml. 



Hematoxylin solution: 



hematoxylin 0.5 gm. 



distilled water 50.0 ml. 



When dissolved, add: 



potassium chromium sulfate (chrome alum) 3%, 



(1.5 gm./50 ml. water) 50.0 ml. 



Mix well and add: 



potassium dichromate, 5% (5 gm./lOO ml. 



water) 2.0 ml 



N/2 sulfuric acid (about 2.5 ml./lOO ml. water) 2.0 ml. 



Ripen for 48 hours. Can be used as long as a film with a metallic 

 luster forms on its surface after 1 day's standing. Filter before use. 



