Clyrogen 301 



3. Coimters tains: 



for nuclei — hematoxylin 



for glycogen — fast green FCF as in Feulgen method 



for mucin or acid polysaccharides — an acid dye (fast green) 



for other polysaccharides — a basic dye (malachite green) 



4. If it is necessary to remove the PAS from the tissue, treat it Avith 

 potassium permanganate until all the color is removed, followed 

 by oxalic acid bleaching of the permanganate. 



Glycogen^ 



Bauer-Feulgen Reaction 



fixation: avoid aqueous media (see McManus and Mowry, 1958). 



absolute eth)l alcohol 9 parts. 



formalin 1 part. 



Start dehydration for embedding in 95% alcohol. Mount slides with 

 95% alcohol. 



solutions: 



Chromic acid: 



chromic acid, CrOg 4.0 gm. 



distilled water 100.0 ml. 



Schiff's reagent, see page 294. 

 Sodium bisulfite: 



sodium bisulfite, meta, NaoSoOj 1.0 gm. 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgClo if present. 



2. Oxidize in chromic acid: 1 hour. 



3. Wash in running water: 5 minutes. 



4. Treat with Schiff's reagent: 10-15 minutes. 



5. Transfer through sodium sulfite, 3 changes: 1, 2, and 2 minutes 

 (see Comment 4, page 295). 



6. Wash in running water: 5 minutes. 



7. Counterstain in hematoxylin, if desired. 



8. Wash and blue in Scott's, and wash, 



9. Dehydrate, clear, and mount. 



See also Rcsl's C'aiiiiinc reaction for glycogen, page 2()7. 



