302 Feulgen and PAS Technics (chap. 19) 



results: 



glycogen — red 

 nuclei — blue 



Desoxyribonucleic Acid, Polysaccharides and Proteins 



Triple Stain (himes and moriber, 1956) 

 fixation: any good general fixative. 



solutions: 



N HCl, see page 408. 

 Azure /1-Schiff reagent : 



*o^ 



azure A (azure I), C.I. 52005 0.5 gm. 



bleach solution (below) 100.0 ml. 



Lasts several weeks, but add a few drops of 10% potassium metabisul- 

 fite before reusing. 



Bleach: 



potassium or sodium metabisulfite, 10% aque- 

 ous 5.0 ml. 



N HCl 5.0 ml. 



distilled water 90.0 ml. 



Make up fresh each time. 



Periodic acid: 



periodic acid (HIO4) 0.8 gm. 



0.2M sodium acetate 10.0 ml. 



distilled water 90.0 ml. 



Make up fresh each time. 



Basic fuchsin-Schiff's reagent, page 294. 



Naphthol yellow S: 



Stock solution: 



naphthol yellow S, C.I. 10316 1.0 gm. 



acetic acid, 1% aqueous 100.0 ml. 



Working solution: 



stock solution 2.0 ml. 



acetic acid, 1% aqueous 100.0 mi. 



Keeps indefinitely. 



