Desoxyribonucleic Acid, Polysaccharides and Proteins 303 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgCL. 



2. Hydrolyze in N HCl, 60°C: 12 minutes, no more. 



3. Rinse in distilled water. 



4. Treat wath Azure ^-Schiff: 5 minutes. 



5. Rinse in distilled water. 



6. Bleach, 2 changes: 2 minutes each. 



7. Rinse in distilled water. 



8. Oxidize in periodic acid: 2 minutes. 



9. Rinse in distilled water. 



10. Treat with basic fuchsin-Schiff: 2 minutes. 



1 1. Rinse in distilled water. 



12. Bleach, 2 changes: 2 minutes each. 



13. Rinse in distilled water. 



14. Stain in Naphthol yellow: 2 minutes. 



15. Rinse briefly in distilled water. 



16. Dehydrate in tertiary or isopropyl alcohol, 2 changes. 



17. Clear and mount. 



results: 



nuclei — blue to green (DNA) 

 polysaccharides — red 

 proteins — yellow 



