Bacteria Staining ^09 



cytoplasm — yellowish 



red blood corpuscles — yellow or reddish yellow 



cartilage — pink 



basophilic granules — red 



comments: 



The author finds it easier to differentiate step 8 by using a coplin jar 

 of ether-acetone (1:3) and dunking the slide. 



Glynn Method (1935) 



fixation: Zenker's without acetic acid preferred. 



solutions: 



Carbol-crystal violet: 



crystal violet, C.I. 42555 (gentian violet) 1.0 gm. 



phenol (carbolic acid) crystals 1.0 gm. 



Work together in mortar, add 



absolute ethyl alcohol 10.0 ml. 



distilled water 100.0 ml. 



Allow to stand 48 hours. Filter. 



Lugol's solution, see page 410. 

 Basic ftichsin: 



basic fuchsin, C.I. 42500 0.05 gm. 



HCl .05N or acetic acid .IN (page 408) 100.0 ml. 



Solution should be pH 2-3. 



procedure: 



1. Deparaffinize and hydrate to water; remove HgCU. 



2. Stain in carbol-crystal violet: 2 minutes. 



3. Drain, but do not wash. 



4. Treat with Lugol's solution: 1 mintue. 



5. Flush with acetone from dropping bottle luitil no more color is 

 removed: 10-15 seconds. 



f). Rinse in distilled water. 



7. Stain in basic fuchsin: 3 mintites. 



8. Drain, but do not wash. 



!). Stain in saturated aqueous picric acid: 30 seconds to 1 miniue. 



10. Wash in running water till sections are light yellow. 



11. Differentiate (some red is lost in sections) and dehydrate in ace- 

 tone: 10-15 seconds. 



12. Xylene and mount. 



