Bdclcrin Slnining^ 311 



cell nuclei — red 



cytoplasm, fibrin, collagen — pink 



comments: 



For tubercle and lepra bacilli it may be necessary to stain for a lonoer 

 period or heat for 90 seconds on a 50-52 °C hot plate. 



MacCallum-Goodpasture Method (mallorv, 1944) 

 fixation: any good general fixative. 



solutions: 



Goodpasture's fuchsin: 



basic fuchsin, C.I. 42500 0.59 gm. 



aniline oil 1.0 ml. 



phenol (carbolic acid) I.O gm. 



or melted 1.0 ml. 



30% alcohol 100.0 ml. 



Sterling's gentian violet: 



crystal violet, C.I. 42500 (gentian violet) 5.0 gm. 



95% ethyl alcohol 10.0 ml. 



aniline 2.0 ml. 



distilled water 88.0 ml. 



Mix aniline and water and filter. Add crystal violet dissolved in alco- 

 hol. Keeps several ^veeks to months. 



Gram's iodine, see page 410. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgClo. 



2. Stain in Goodpasture's fuchsin: 10-20 minutes. 



3. Wash in water. 



4. Differentiate in formalin (full strength): few seconds. Red color 

 changes to rose. 



5. Rinse in distilled water: few seconds. 



6. Treat with picric acid, saturated aqueous: 3-5 miniues. Sections 

 turn purplish yellow. 



7. Wash in distilled w^ater: few seconds. 



8. Differentiate in 95% alcohol. Sections turn red; some of it washes 

 out, also some of yellow. 



9. Rinse in distilled water. 



10. Stain in Sterlino's oentian violet: .3-5 minutes. 



1 1. Rinse in distilled water. 



12. Treat with Gram's iodine: 1 minute. 



