Fimgi Staining 321 



solutions: 

 Periodic acid: 



periodic acid 1.0 gm. 



distilled water 100.0 ml. 



SchifF's reagent, see page 294. 



Differentiator: 



potassium metabisulfite, 10% aqueous 5.0 ml. 



N HCl (page 408) 5.0 ml. 



distilled water 100.0 ml. 



Light green: 



light green SF yellowish, C.l. 42095 0.2 gm. 



distilled water 100.0 ml. 



glacial acetic acid 0.2 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgCU if present, 



2. Oxidize in periodic acid: 5 minutes. 



3. Wash in riuming; water: 15 minutes. 



'O 



4. Treat with Schiff's reaoent: 10-15 minutes 



O' 



5. Differentiate, 2 changes: total 5 minutes. 



6. Wash in rimning water: 10 minutes. 



7. Stain in light green: 3-5 minutes. If too dark, rinse in running 

 water. 



8. Dehydrate, clear, and mount. 



results: 



fungi — ^red. Not specific, however — glycogen, mucin, amyloid, colloid 



and others may show rose to purplish red 

 backgTOund — light green 



comments: 



To remove glycogen, starch, mucin, or RNA, see page 300. 



Gridley Method (1953) 



fixation: anv oood oeneral fixative. 



; o 



solutions: 



Chromic acid: 



chromic acid 4.0 gm. 



distilled water 100.0 ml. 



Schiff reagent, see page 294. 



