Fungi Staining 323 



Light green, Stock solution: 



light green SF, yellowish, C.l. 42095 0.2 gm. 



distilled water 100.0 ml. 



glacial acetic acid 0.2 ml. 



Working solution: 



light green stock solution 10.0 ml. 



distilled water 100.0 ml. 



procedure: 



1. Deparaffinize and hydrate slides to water; remove HgCU. 



2. Oxidize in periodic acid, 0.5% (0.5 gm./lOO ml. water): 10 min- 

 utes. 



3. Wash in running water: 3 minutes. 



4. Oxidize in chromic acid, 5% (5 gm./lOO ml. water): 45 minutes. 



5. Wash in running water: 2 mintues. 



6. Treat with sodium bisulfite, 2% (2 gm./lOO ml. water): 1 minute 

 to remove chromic acid. 



7. Wash in rimning water: 5 minutes. 



8. Rinse in distilled water, 2-3 changes: 5 minutes total. 



9. Place in methenamine silver nitrate, 58°C: 30 minutes. Do not 

 use metal forceps. Sections appear yello^vish brown. 



10. Wash thoroughly, several changes distilled water. 



11. Tone in gold chloride (10 ml. stock soliuion/90 ml. water) until 

 sections turn purplish grey, fungi are black. 



12. Rinse in distilled water. 



13. Fix in sodium thiosulfate, 5% (5 gm./lOO ml. water): 3 minutes. 



14. Wash in running water: 5 miniues. 



15. Coimtcrstain in light green: 30 seconds. 



16. Dehydrate, clear, and mount. 



results: 



fimgi — black 

 backgrotuid — light green 



comments: 



Mowry's method tises oxidation with both periodic and chromic acid 

 (former methods tise only the latter) with the result that the final 

 staining is stronger and m®re consistent than that of the original 

 Gomori method. 



Fluorescent Method (fickett et al., 1960) 

 iixAtiox: 10% formalin, Zenker's, alcohol and others. 



