Mounting Media 355 



it dilutes the xylene to a minimum.) The cover glasses tend to slip, 

 so seal them with one of the ringing cements (page 123). 



Mounting Media 



Burstone {1957 A) recommends for some azo-dye methods: 



1. Mount directly from 75-80% alcohol in 20% solution of polyvinyl 

 acetate {Gelva 2.'^, Shaivinigan Resins Corporation, Springfield, 

 Massachusetts) in 80%, alcohol. It is soluble also in glacial acetic 

 acid, //-butanol (90%), aniline, pyridine, and ethyl cellosolve. 



2. It preserves the follo^ving: 



esterase, alkaline phosphatase, PAS, hematoxylin, celestin blue, 

 aldehyde fuchsin, eosin. 



3. Cover glass is immovable after 1 hour. No bubble formation. In- 

 dex of refraction, 1.3865, but increases after evaporation of alcohol 

 and water. 



