Exfoliative Cytology 357 



After fixation, the slides should not be allowed to dry at any time 

 before or during the staining procedure. In emergencies, they can be 

 shipped dry, but if possible some protection for the smear is advisable. 

 Ehrenrich and Kerpe {1959) add 5% of polyethylene glycol to the fixa- 

 tive. Following complete fixation, allow the slides to dry 5-10 minutes, 

 and then prepare them for shipping. Papanicolaou {1957) protects 

 smears for shipping by covering the fixed smears with Diaphane^ solu- 

 tion: 2 parts of Diaphane to 3 parts of 95% alcohol. AIIoav to dry, 20-30 

 minutes, and prepare for shipping. The polyethylene glycol or the 

 Diaphane forms a protective coating over the smear. 



Richardson {I960) uses the following solution for shipping purposes; 

 it is economical, noncombustible, and nonvolatile. Either biopsy pieces 

 or slides can be shipped in it. 



monoethylene glycol 344.6 ml. 



diethylene glycol 18.1 ml. 



borax 3.6 gm. 



water 577.6 ml. 



glacial acetic acid 50.0 ml. 



Papanicolaou Method (1942, 1947, 1954, 1957) 



Excellent preparations of the following solutions can be purchased 

 from the Ortho Pharmaceutical Corporation, Raritan, N.J. (Harris He- 

 matoxylin, EA 65, EA 50 and OF 6). Otherwise the stains can be pre- 

 pared as follows: 



solutions: 



Harris Hematoxylin, page 125 (omit acetic acid). 

 Orange G 6 {OG 6): 



orange G 6, Q.b% (0.5 gm./lOO ml. 957o alco- 

 hol) lOO.O ml. 



phosphotungstic acid 0.015 gm. 



Eosin — azure 36 {EA 36 or EA 50, the commercial designation): 

 light green SF yellowish, C.I. 42095, 0.5% (0.5 



gm./lOO ml. 95% alcohol) 45.0 ml. 



bismarck brown, C.I. 21000, 0.5% (0.5 gm./lOO 



ml. 957o alcohol) 10.0 ml. 



eosin Y, C.I. 45380, 0.5^^ (0.5 gm./lOO ml. 95^^ 



alcohol) 45.0 ml. 



phosphotungstic acid 0.2 gm. 



lithium carbonate, saturated aqueous 1 drop 



^Obtained from ^Vill Corp., Rochester, N.Y. 



