Exfoliative Cytology 361 



nuclei — yellowish hues, yellow-orange to yellow-green 

 nucleoli — brilliant orange-red 

 degenerating and necrotic malignant cells — characterized by a grad- 

 ual loss of cytoplasmic RNA and therefore of the flaming red- 



orange 



cytoplasm — faint orange or brick red, RNA often concentrated 



at periphery in a rim of dark brick red 

 nuclei — brilliant green, green-yellow or pale yellow-gTey 

 nucleoli — often enlarged and multiple, orange-red 



comments: 



Paraffin sections also can be stained by this method. 



The buffer mount is a temporary mount, even when ringed. Upon 

 the author's suggestion, the laboratory at Los Alamos has tried dis- 

 solving Elvanol 51-50-^ in the buffer until the solution is of thin syrup 

 consistency. This has resulted in a clearer and more lasting mount. 

 (See also page 122) Seal the cover slip, how^ever, wath one of the ring- 

 ing cements (page 123). Many scientific supply houses are now adver- 

 tisinsf media for fluorescent mountino-. 



After the smear has been used for fluorescent examination, it can 

 be stained by the Papanicolaou technique. 



1. Remove cover glass in distilled water. 



2. Transfer to 50% alcohol: approximately 1 minute, 2 changes. 



3. Proceed to Papanicolaou method. 



Bacteria and parasites will fluoresce and can confuse the picture; 

 for example: bacteria — bright orange: Trichomonas, cytoplasm — 

 bright orange and nucleus — whitish yelloAV". These are easily recog- 

 nized in vaginal smears. See Urniker and Pickle {I960) for lung can- 

 cer diagnosis. 



Dart and Turner (1959) Method 



solutions: 



Mcllvaine's buffer: 



sodium phosphate, Na2HP04 10.081 gm. 



citric acid, monohydrate 13.554 gm. 



distilled water 1000.0 ml. 



Keep in refrigerator. Good for 1 ^veek. 



Buffered acridine orange: 

 Stock solution. 



acridine orange, C.I. 46005 0.1 gm. 



distilled water 100.0 ml. 



* PVA, polyvinyl alcohol, E. I. DuPont de Xcmours and Co., ^\■ilmington, Del. 



