370 Special Procedures I (chap. 22) 



D. Lamination Method, page 394. 



For additional Staining Methods, see: 



Austin {1959): iron alum modification with acetocarmine. 



Bradley (1937): sudan black with acetocarmine. 



Griffin and McQiiarrie {1942) and Chen {1944): iron hematoxylin. 



Melander and Wingstrand {1953): Gomori's hematoxylin. 



Rafalko {1946) and Sachs {1953): Feulgen methods. 



Carter et al. {1959) recommend aceto-orcein squashing for cancer 



detection; mitotic figures are red and detailed. Sex chromatin is 



recognizable. 



Sandberg et al. {I960) describe in detail preparation of aspirated 



bone marrow for aceto-orcein staining. 



Restoration of deteriorated slides {Persidsky, 1954) 



If dye has precipitated as dark crystals because the preparation has 

 dried: 



1. Remove sealing material. 



2. Place 1 drop of 2N HCl at one edge of cover glass. Apply blotter 

 to opposite edge to help draw the acid imder: 3-5 minutes. 



3. Replace HCl with acetocarmine by same method. 



4. Heat gently, do not boil. Crystals are redissolved and specimen 

 restained. 



5. Reseal. 



References: Austin (1959); Belling (1926); Bradley (1948A&B, 1957); 

 Carter et al. (1959); Celarier (1956); Chen (1944); Conger and Fair- 

 child (1953); GrifTin and McQuarrie (1942); LaCour (1935, 1941); 

 McClintock (1929); Melander and Wingstrand (1953); Moree (1944); 

 Nolte (1948); Peary (1955); Persidsky (1954); Rafalko (1946); Rothfels 

 and Siminovitch (1956); Sachs (1953); Smith (1947) ; Warmke (1935); 

 Zirkle (1937); and Zuck (1947). 



